Tissue-Tek® Cryo3® Flex Cryostat

The Tissue-Tek Cryo3 Flex Cryostat produces superior sections from 1 to 99 microns using a temperature-controlled blade holder and a 3D Precision Chuck to accurately align the block face to the b

Innovative Cryostat technology

View Interactive Brochure

The Tissue-Tek® Cryo3® Flex Cryostat is the 3rd generation of the established, reliable and trusted Cryo3 platform for fast sectioning of frozen tissue specimens. It was designed to decrease the time to get complete sections, and in parallel to preserve valuable tissue.

The Tissue-Tek Cryo3 Flex Cryostat produces superior sections from 1 to 99 microns using a temperature-controlled blade holder and a 3D Precision Chuck to accurately align the block face to the blade, reducing operator’s trimming time and preserving specimens. The sturdy chuck retains its position when locked and unlocked, eliminating any time and effort spent realigning the cutting angle.

You can now leverage the newly developed Anti-Roll Rake that was designed to best mimic the preferred and widely accepted "Brush Technique" to prevent frozen sections from rolling, which in addition, reduces the learning curve of new users making them proficient faster.

For the first time in the history of cryotomy, Sakura Finetek successfully integrated its advanced remote diagnostics technology platform, Tissue-Tek® iSupportTM, into the hardware and software of this frozen section cryostat. The plug-and-play connection opens communication to a secure remote service that ensures maximum uptime through fast reaction to instrument alerts by Sakura Finetek’s professional technical support team.  

The cryostat's color touch LCD screen provides a visual display of critical sectioning information such as current chamber temperature, section thickness, and trimming thickness.  The Cryobar® offers 12 positions, allowing operators to use the Cryomold® method or direct method at the same time.  The Cryo+™ area of the Cryobar freezes to -50C, within 5 minutes after activation.

The motorized advance and retract keys on the cryostat allows smooth, precise orientation of the 3D Precision Chuck to the blade holder. The blade holder accommodates Accu-Edge® high and low-profile disposable blades.

The Cryo3 Flex D and DM models offer innovative options like motorized frozen sectioning, ozone disinfection, and a vacuum debris removal system. The Tissue-Tek Cryo3 Flex Cryostat now uses a more efficient and patented ozone technology to safely protect operators from pathogens in between weekly decontamination requested by CAP Accreditation Guidelines. The combination of ozone, UV and elevated temperatures provide unprecedented disinfection with a reduction of up to 5 logs for the SV40 virus; Learn more about the use of ozone for inactivation of bacteria and viruses in cryostats.



3D Precision Chuck
  • Obtain complete sections faster
  • Retains position when locked and unlocked
  • Quick and precise fine angle adjustments on all 3 axes (XYZ)
1" Travel distance
  • Cut thicker specimens
Utility Tray with storage space
  • Store up to 32 specimen holders in the chamber to keep as many blocks frozen for immediate recuts
2 Optional Ergonomic Handles
  • Section more comfortability
  • Help prevent Repetitive Motion Disorder (RMD)
Tissue-Tek® iSupportTM: online report diagnostics technology
  • Maximize instrument uptime
  • Get instrument support faster
Ozone disinfection
(D and DM models)
  • Protect yourself from pathogens with an unprecedented disinfection of up to 5 logs for the SV40 virus
Anti-Roll Rake
  • Prevent sections from rolling
Product names Tissue-Tek® Cryo3® Flex Cryostat, Basic
Tissue-Tek® Cryo3® Flex Cryostat, D
Tissue-Tek® Cryo3® Flex Cryostat, DM
Product codes 6200
Application Cryotomy
Dimensions 25.8 (W) x 29.7 (D) x 47.0 (H) inches
65.4 (W) x 75.4 (D) x 119.4 (H) cm
Weight 373 lbs (169.2 kg)
Power requirement 115/120 VAC, 50/60 Hz, 11 A
The instrument must be connected to an electrical outlet which supplies the proper voltage (within ± 10%)
Data interface USB and LAN
Chamber temperature
set point range
-35°C to 0°C; Chamber temperature is maintained within ±2°C
Cryo+ temperature When activated Cryo+™ is 20°C lower than the Chamber temperature, within 5 minutes. Lowest Cryo+™ temperature is -50°C
Section thickness settings 1 to 99 microns in 1 micron steps
Maximum specimen thickness 25 mm
Trimming range 1 to 100 microns in 1 micron steps
Specimen Holder sizes Round 26 mm, 36 mm, 55 mm
(all including an alignment mark on the shaft)
Specimen orientation ±8° (XY axis), 360° (Z axis)
Chuck 3D Precision Chuck secures the Specimen Holder to the Microtome. Dial indicators show the exact angle of the Chuck. The guide marks show the exact orientation of the Specimen Holders in the Chuck
Travel range Vertical: 2.5 inches (63.0 mm)
Horizontal: 1.0 inch (25.4 mm)
Defrost cycle Manual start: defrosts the Chamber within 30 minutes, when activated
Automatic start: defrosts the Chamber within 1 hour; start time can be set within a 24 hour period
Disinfection (D and DM models only) Manual start: two Ozone Disinfections cycles are available:
• Long: approximately 135 minutes (warm)
• Short: approximately 45 minutes (cold)
Automatic start: always performs a Long Disinfection Cycle.
Start time can be set within a 24 hour period
Certifications IEC 61010-1:2010 Ed:3, IEC 61010-2-010 Ed:3, IEC 61010-2-101 Ed:2, UL 61010-1 Ed:3, CAN/CSA C22.2 # 61010-1:2012 Ed:3, EMC: CENELEC IEC/EN 61326-1, EMC: CANELEC IEC/EN 61326-2-6
Regulatory status IVD, FDA Class I

Product code

Product name and quantity

4583 Tissue-Tek® O.C.T. Compound; 12/case
4685 Accu-Edge® High Profile Microtome Blades; 500/case
4689 Accu-Edge® Low Profile Microtome Blades; 500/case
4728 Cryomold® Standard; 23 mm; 1,200/case
4730 Cryomold® Biopsy; 15 mm; 1,200/case
6033 Uninterrupted Power Supply (UPS), 30 minutes 

MWEB0105 Rev.F

Back to top