Thick and thin: Part of the section is thick, and part of the section is thin, reflecting inconsistent staining.
Causes
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Remedies
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Locking mechanisms loose on microtome
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- Verify locking mechanisms are tight
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Blade tension too tight
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- Loosen tension on the blade
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Dull blade
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- Move blade to an unused area or insert a new blade
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Sectioning too quickly
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- Slow down sectioning speed
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Blade angle too low
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- Increase blade angle at 1-degree increments
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