Moth holes/Why are there holes in tissue sections?

Moth holes:  parallel lines in the tissue or separation of tissue into fine lines


Causes

Remedies

Overagressive trimming- too fast, too thick, or both
  • Slow down trimming speed, and reduce trimming micron thickness

Block too cold
  • Do not let sit on ice too long; lightly warm the block with your finger

Poor tissue processing
  • Soak block on ice bath to soften overprocessed, hard tissue
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